The use of polymerase chain reaction generated nucleotide sequences as probes for hybridization
SP Scully, ME Joyce, N Abidi, ME Bolander - Molecular and cellular probes, 1990 - Elsevier
In this report a rapid, simple and economical means of preparing a cDNA probe of specified
length, sequence and specific activity is described. The process involves the use of a
polymerase chain reaction to incorporate radiolabelled nucleotides into a single stranded or
double stranded cDNA sequence. A pair of oligonucleotide primers are synthesized,
flanking the sense and antisense strands of a selected sequence. The primers are then used
with a cloned DNA fragment or a cellular source of RNA or DNA as a template to amplify the …
length, sequence and specific activity is described. The process involves the use of a
polymerase chain reaction to incorporate radiolabelled nucleotides into a single stranded or
double stranded cDNA sequence. A pair of oligonucleotide primers are synthesized,
flanking the sense and antisense strands of a selected sequence. The primers are then used
with a cloned DNA fragment or a cellular source of RNA or DNA as a template to amplify the …
