[HTML][HTML] Recombinant phospholipase Cζ has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs
Z Kouchi, K Fukami, T Shikano, S Oda… - Journal of Biological …, 2004 - ASBMB
Sperm-specific phospholipase Cζ (PLCζ) is known to induce intracellular Ca 2+ oscillations
and subsequent early embryonic development when expressed in mouse eggs by injection
of RNA encoding PLCζ (Saunders, CM, Larman, MG, Parrington, J., Cox, LJ, Royse, J.,
Blayney, LM, Swann, K., and Lai, FA (2002) Development 129, 3533-3544). The present
study addressed characteristics of purified mouse PLCζ protein that was synthesized using
the baculovirus/Sf9 cell expression system. Microinjection of recombinant PLCζ protein into …
and subsequent early embryonic development when expressed in mouse eggs by injection
of RNA encoding PLCζ (Saunders, CM, Larman, MG, Parrington, J., Cox, LJ, Royse, J.,
Blayney, LM, Swann, K., and Lai, FA (2002) Development 129, 3533-3544). The present
study addressed characteristics of purified mouse PLCζ protein that was synthesized using
the baculovirus/Sf9 cell expression system. Microinjection of recombinant PLCζ protein into …