Glass adherence assay. (a–c) Assay was performed for 3 hours in DMEM–0.5% glucose with 0.1 mM IPTG at 37°C in atmosphere. (a) 042; (b) 042aap; (c) 042aap(pAap). (d) Quantitation of adherence grown in DMEM without IPTG (042 and 042aap) or with the indicated concentration of IPTG [(042aap(pAap)]. Bacteria were removed from glass using 1% Triton X-100 and quantitated by viable count. Assays were performed in triplicate, and means are illustrated in histograms with error bars indicating SEM. *P < 0.05 vs. 042aap by one-tailed t test of log-transformed data.